Transmembrane transport of anionic fluorescent dyes by suspension-cultured plant cells. by Anne Kearns

Cover of: Transmembrane transport of anionic fluorescent dyes by suspension-cultured plant cells. | Anne Kearns

Published by Oxford Brookes University in Oxford .

Written in English

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Edition Notes

Thesis (M.Phil.) - Oxford Brookes University, Oxford, 1996.

Book details

ContributionsOxford Brookes University. School of Biological and Molecular Sciences.
The Physical Object
Pagination129 leaves :
Number of Pages129
ID Numbers
Open LibraryOL19180217M

Download Transmembrane transport of anionic fluorescent dyes by suspension-cultured plant cells.

Plasma membrane anion transporters play Transmembrane transport of anionic fluorescent dyes by suspension-cultured plant cells. book roles in plant cell biology, especially in stomatal closure and nutrition. Notwithstanding, a lot is still unknown about the specific function of these transporters, their specific localization, or molecular nature.

Here the fundamental roles of anionic transport in plant cells are by: A series of fluorescent anion transporters consisting of a urea or thiourea group linked to a naphthalimide fluorophore have been synthesised and their anion transport properties studied.

The compounds possess similar anion transport properties to (thio)urea-based anionophores that have previously been repor ISACS Challenges in Organic Materials and Supramolecular Cited by:   Abstract. The charging of the plasma membrane is a necessary condition for the generation of an electric-field-induced permeability increase of the plasmalemma, which is usually explained by the creation and the growth of aqueous pores.

For cells suspended in physiological buffers, the time domain of membrane charging is in the submicrosecond by: 5. Chapter 11 – Transmembrane Transport of Ions and Small Molecules. Bacterial aquaporin: Transports water (blue) and glycerol (not shown) into and out of the cell. Four identical monomers (shown embedded in a phospholipid membrane [yellow]) Each monomer has a water channel through its center.

A series of fluorescent anion transporters consisting of a urea or thiourea group linked to a naphthalimide fluorophore have been synthesised and their anion transport properties studied.

The compounds possess similar anion transport properties of (thio)urea-based anionophores that have previously been : Stuart Berry, Vanessa Soto-Cerrato, Ethan Howe, Harriet Clarke, Ishna Mistry, Ali Tavassoli, Young-T.

Like in S2 cells, organelles in cultured neurons can be visualized by either organelle-specific fluorescent dyes or by using fluorescent organelle markers encoded by. We describe here the application of a carbocyanine dye, 3,3′-dipropylthiodicarbocyanine iodide [DiS-C 3-(5)], to monitor the transmembrane potential changes induced by a variation of the K + concentration for the cells of Escherichia (E.) coli and photosynthetic bacterium Rhodospirillum (R.) Cited by: Tetrahedron Letters,Vol,No,pp/85 $ + Printed in Great Britain c Perciamon Press Ltd.

CHARGE TYPE AND TRANSEMBRANE TRANSPORT OF FLUORESCENT PROBES IN SURFACTMT VESICLES Robert A. Moss,* Shanti Swarup, Boguskawa Wilk, and Thomas F. Hendrickson Department of Chemistry, Rutgers, The State Cited by: 1. The organic anion transport inhibitor, probenecid, inhibits the transport of lucifer yellow at the plasma membrane and the tonoplast in suspension-cultured plant cells.

Eukaryotic cells are electronegative when compared to the surrounding environment. This negative charge Transmembrane transport of anionic fluorescent dyes by suspension-cultured plant cells.

book called the transmembrane potential (TMP) and is partly caused by concentration gradients of K +, Na 2+, and Cl − ions across the cell membrane. For purposes of cell osmolarity and pH balance, Na 2+ and Cl − ion concentrations are kept lower inside the cell Cited by: 7.

In addition, the fluorescent dyes FM and FM have been successfully used to study the endocytic pathway in plant cells. After their insertion in the PM, these styryl dyes pass through endosomal compartments on their way to the tonoplast (Vida and Emr,Kim et al., ; Ueda et al., ; Emans et al., ; Tse et al., Cited by: Fluorescent Kits for Cell Biology Active Motif, through its Active Motif Chromeon division, strives to develop innovative cell biology assays that incorporate its proprietary fluorescent dyes, conjugates and target-specific fluorescent probes.

Planr Phwiol. Biochrm., 36 (12). Establishment of low extracellular pH is essential for uptake of the fluorescent anionic dye hydroxypyrenetrisulfonate by suspension- cultured carrot cells Anne Kearns, Louise Cole, Chris R. Hawes, David E.

Evans* Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Headington, Cited by: 2. Inactivation of a fluorescent dye in a very concentrated spot on a cell so that the fluidity of membranes can be visualized is called.

You are experimenting with a variety of lipids to determine their efficacy for use as drug delivery system liposomes. The marine plathyhelminth Macrostomum lignano was recently isolated from Adriatic shore sediments where it experiences a wide variety of environmental challenges, ranging from hypoxia and reoxygenation, feeding on toxic algae, to exposure to anthropogenic contaminants.

As multidrug resistance transporters constitute the first line of defense against toxins and Cited by: 5. Useful for tissue sections, in vivo neurons, fixed tissue sections and cultured cells, these lipophilic neuronal tracers are used in both anterograde and retrograde transport studies.

Carbocyanine dyes, such as DiI and its derivatives, label cell membranes without appreciably affecting cell viability and can be used for long-term studies.

The dyes uniformly label neurons via lateral. Polarity sensitive dyes are powerful tools to characterize such lipid membrane order.

These dyes change their emission spectrum depending on the polarity of the environment which can be used to quantify the molecular ordering and to visualize lateral heterogeneity in membrane order of cellular membranes. Low PS, Heinstein PF. Elicitor stimulation of the defense response in cultured plant cells monitored by fluorescent dyes.

Arch Biochem Biophys. Sep; (2)– May JM, de Haën C. Insulin-stimulated intracellular hydrogen peroxide production in rat epididymal fat cells. J Biol Chem. Apr 10; (7)–Cited by: [Hydrophobic acridine dyes for fluorescent staining of mitochondria in living cells.

Specific accumulation of the fluorescent dye NAO on the mitochondrial membranes in HeLa cells by hydrophobic interaction. Depression of respiratory activity, changes in the ultrastructure of mitochondria due to by: In cellular biology, membrane transport refers to the collection of mechanisms that regulate the passage of solutes such as ions and small molecules through biological membranes, which are lipid bilayers that contain proteins embedded in them.

The regulation of passage through the membrane is due to selective membrane permeability - a characteristic of biological. Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes Anthony Mathur a The Cell Biology Group, Centre for Vascular Biology and Medicine, Department of Medicine, University College London, Room G15, Rayne Institute, 5 University Street, London WC1E 6JJ, UKCited by: The slow-response potential-sensitive probe, DiBAC 4 (3) enters depolarized cells where it binds to intracellular proteins or membrane and exhibits enhanced fluorescence and a red spectral shift.

Increased depolarization results in additional influx of the anionic dye and an increase in fluorescence. Cajal bodies are found in the nuclei of plant and animal cells, and the number and size of Cajal bodies per cell are subject to change, depending on cell type and cell cycle (Gall, ; Ogg and Lamond, ).

Cajal bodies are very often located at the nucleolar periphery and even within nucleoli. Because of their hollow interior, transmembrane channels are capable of opening up pathways for ions across lipid membranes of living cells.

Here, we demonstrate ion conduction induced by a single DNA duplex that lacks a hollow central channel. Decorated with six porpyrin-tags, our duplex is designed to span lipid membranes.

Combining electrophysiology measurements with Cited by: Abstract. Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue ® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune cell functions in vitro and in include: migration and adhesion; proliferation of stem and progenitor cells; differentiation and growth control; mechanisms of.

A series of easy-to-make fluorinated tripodal anion transporters containing urea and thiourea groups have been prepared and their anion transport properties studied. Vesicle anion transport assays using ion-selective electrodes show that this class of compound is capable of transporting chloride through a lipid bilayer via a variety of mechanisms, including chloride/H+ cotransport Cited by: Cell Culture and Fluorescent Staining Page 1 Culturing and Fluorescent Staining of B16 Melanoma Cells In this lab exercise you will learn how animal cells can be grown in culture and used to analyze the cytosolic arrangement of actin filaments.

Cell culture is a File Size: KB. Aquaporin-1, an AQP in human red blood cells, is the first reported water facilitator that speeds up transmembrane influx and efflux of water. Since then, more AQP and GLP homologues have been found and extensively studied in mammals and plants [11,12,13,14,15,16].

eqFP can be functionally expressed in plant cells. Recently, eqFP, the gene for a red fluorescent protein from the sea anemone Entacmaea quadricolor, has been cloned and characterized [13, 14].This protein has been succesfully expressed in bacteria and animal cells [], but has not yet been tested in test its use as a marker in plants, the Cited by: Fluorescent Dyes Edward Leber Cell surface sialic acids N 3 Intracellular O-GlcNAc N 3 Feed cells 1–3 days N 3 O-linked cell surface and intracellular O-GlcNAc Ac 4ManNAz AcO O AcO OAc HN O OAc N3 Jacobs CL, Yarema KJ, Mahal LK, Nauman DACharters NW, Bertozzi CR; Methods Enzymol.

; Modeling of ion transport via plasma membrane needs identification and quantitative understanding of the involved processes. Brief characterization of main ion transport systems of a yeast cell (Pma1, Ena1, TOK1, Nha1, Trk1, Trk2, non-selective cation conductance) and determining the exact number of molecules of each transporter per a typical cell allow us to Cited by: JPA JPA JPA JPA JP A JP A JP A JP A JP A JP A JP A Author: ゴンザレズ,ジーザス,イー.,サード, ツィエン,ロジャー,ワイ..

• Transmembrane carrier protein, found in the plasma membrane of most animal cells, that pumps Na+ out of and K+ into the cell, using the energy derived from ATP hydrolysis. Osmosis • Osmosis is the movement of water molecules through a selectively-permeable membrane down a water potential gradient.

a transport protein in the plasma membrane of animal cells that actively transports sodium out of the cell and potassium into the cell exocytosis When the golgi vesicle fuses with the cell membrane, and expels its contents.

Cells were pre-labeled for 1 h with 1 μ m Spd-C 2-BODIPY followed by a min incubation with LysoTracker Red (LTR). The fluorescent probes were then removed and cell cultures were incubated for 0, 30, 60 and 90 min with μ m bafilomycin A 1 (B) or vehicle only (% Me 2 SO, v/v) (A).

DHI Cell Culture and Fluorescent Tests Quidel is the largest manufacturer of cell culture products in the world. Quidel offers many conventional and proprietary cell line products under the Diagnostic Hybrids (DHI) brand for use in viral detection and identification.

The real-time path of cell migration can be monitored and the absolute speed of cell migration can be determined by high throughput living image system. Figure 1: The results of Fluorescent-labeled Cell Migration Assay. Since this method is based on the fluorescent label to study cell migration, it also called fluorescent-labeled cell migration.

The Ca 2+ ‐sensitive fluorescent dye acetoxymethyl ester Fluo‐3 (cell‐permeant) for imaging live‐cell intracellular Ca 2+ levels, and the potentiometric probe DiBAC 4, an anionic oxonol, suitable for a noninvasive monitoring of the PM potential, were purchased from Molecular Probes (Eugene, OR, USA).Cited by: Are you testing fluorescent dyes to test their permeability in healthy, non-apoptotic cells.

Or are you looking for a dye that only enters the cell under cell permeabilized conditions. DAPI is excluded from live, non-apoptotic cells. Hoeschst stain is very similar to DAPI. These are only taken up when cells have been permeabilized, such as when.

cell membrane and is subsequently hydrolyzed inside of the cells by intracellular esterases. The resulting probe is a hydrophilic fluorescent dye that is trapped within the cell unless actively pumped out by an ABC transporter (Fig 1).

The fluorescence signal of the dye generated within the cells thus depends upon the activity of the ABC transport. Molecular Targeting Technologies, Inc. (PS) exposed on cell membranes.

The fluorescent part of the probe is a reporter element that provides a means of detecting the probe once it is bound to the membrane of interest.

Key Features of PSVue dye solubility and is suitable for in vitro and in vivo use.Selectivity. In the competition of bacterial cells and host cells with the antimicrobial peptides, antimicrobial peptides will preferentially interact with the bacterial cell to the mammalian cells, which enables them to kill microorganisms without being significantly toxic to mammalian cells.fluorescent dyes and drugs can encapsulated within the stabilized liposomes without leakage.

12 Liposomes change phase when heated or cooled to their phase-transition temperature. Liposomes may be heated by external stimulation tp allow release of their contents. Moreover, the combination of different treatment methods (e.g.

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